Welcome to our lab website!

The ABCD Lab consists mainly of physicists working in tight collaboration with biologists and chemists. Our strong experimental background enabled the development of new reliable tools to study different problematics in biology. Today, the activities of the lab encompass approximately all scales of description in biology: single molecule micromanipulation, study of replication fork rescue in single bacterium, cell fate addressing during development of zebrafish and phenotypic variability in bacterial population.
 

1. Single molecule micromanipulations are devoted to the study of DNA and DNA-protein interactions based on a magnetic tweezers system. The lab is currently working at:
       – an in-vitro reconstitution of the replication fork
       – a new sequencing method of single DNA molecules
       – the action of diverse helicases, topoisomerases and translocases on DNA
2. At the cell level, the lab is interested in how in-vivo replication forks can restart after controlled arrest. This project involves single molecule fluorescence and microfluidics.
3. The part of the lab dedicated to developmental biology uses a 2-photon set-up to influence cell lineage in the course of embryo development by using photo-activable molecules which can be rapidly triggered in a single cell at a given time.
4. At the population level, the lab is focusing on the role of phenotypic variability for iron regulation in Pseudomonas aeruginosa population. The technics developed for this purpose are essentially: microscopy, image analysis, microfluidics and laser ablation. We are also trying to implement a long term evolution experiment of Escherichia coli in a temperature gradient.

Postal adress: 24, rue Lhomond 75005 Paris
Phone number: +33 (0)1 44 32 34 92
Fax number: +33 (0)1 44 32 34 33