ALIGNMENT AND SENSITIVE DETECTION OF DNA BY A MOVING INTERFACE

Publication Type:

Journal Article

Source:

SCIENCE, AMER ASSOC ADVANCEMENT SCIENCE, Volume 265, Number 5181, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005, p.2096-2098 (1994)

Keywords:

ASSEMBLIES; GEL-ELECTROPHORESIS; HYBRIDIZATION; MAGNETIC BEADS; MICROSCOPY; MOLECULES; NUCLEIC-ACIDS

Abstract:

In a process called `'molecular combing,'' DNA molecules attached at one end to a solid surface were extended and aligned by a receding air-water interface and left to dry on the surface. Molecular combing was observed to extend the length of the bacteriophage lambda DNA molecule to 21.5 +/- 0.5 micrometers (unextended length, 16.2 micrometers). With the combing process, it was possible to (i) extend a chromosomal Escherichia coli DNA fragment (10(6) base pairs) and (ii) detect a minute quantity of DNA (10(3) molecules). These results open the way for a faster physical mapping of the genome and for the detection of small quantities of target DNA from a population of molecules.