Estimating the persistence length of a worm-like chain molecule from force-extension measurements

Publication Type:

Journal Article

Source:

BIOPHYSICAL JOURNAL, BIOPHYSICAL SOCIETY, Volume 76, Number 1, Part 1, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA, p.409-413 (1999)

Keywords:

DNA-MOLECULES; ELASTICITY; TITIN; TWEEZERS

Abstract:

We describe a simple computation of the worm-like chain model and obtain the corresponding force-versus-extension curve. We propose an improvement to the Marko and Siggia interpolation formula of Bustamante et al (Science 1994, 265:1599-1600) that is useful for fitting experimental data. We apply it to the experimental elasticity curve of single DNA molecules. Finally, we present a tool to study the agreement between the worm-like chain model and experiments.