Micelles of lipid-oligonucleotide conjugates: Implications for membrane anchoring and base pairing

Publication Type:

Journal Article

Source:

JOURNAL OF PHYSICAL CHEMISTRY B, AMER CHEMICAL SOC, Volume 108, Number 20, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA, p.6485-6497 (2004)

DOI:

10.1021/jp031188m

Keywords:

2ND-HARMONIC GENERATION; CARBONYL-COMPOUNDS; CRUMPLING TRANSITION; DNA; FLUORESCENCE CORRELATION SPECTROSCOPY; MOLECULAR ARCHITECTURE; OLIGODEOXYNUCLEOTIDES; POLYMERIZATION; SYSTEMS; VESICLES

Abstract:

This report examines the organization properties of new fluorescent DNA-lipids, either alone in water or in interaction with 1-otyl-beta-D-glucopyranoside micelles or egg phosphatidylcholine vesicles. We first describe the design and the syntheses of the conjugates. Then, we use UV-Vis absorption, steady-state fluorescence emission, electron microscopy, and fluorescence correlation spectroscopy after two-photon excitation to show that these DNA-lipids form spherical micelles in aqueous solution and incorporate much better in micelles than in vesicles. We also investigate the significance of the lipophilic chains of these DNA-lipids on the melting behavior of the double-stranded hybrids: in water melting curves are broadened whereas in amphiphilic assemblies duplexes melt as the unconjugated controls. This work is expected to be useful for improving the rational design of antisense medicines.