Optical control of protein activity and gene expression by photoactivation of caged cyclofen.

Publication Type:

Journal Article

Source:

Methods in enzymology, Volume 624, p.1-23 (2019)

DOI:

10.1016/bs.mie.2019.04.009

Abstract:

The use of light to control the expression of genes and the activity of proteins is a rapidly expanding field. While many of these approaches use a fusion between a light activatable protein and the protein of interest to control the activity of the latter, it is also possible to control the activity of a protein by uncaging a specific ligand. In that context, controlling the activation of a protein fused to the modified estrogen receptor (ERT) by uncaging its ligand cyclofen-OH has emerged as a generic and versatile method to control the activation of proteins quantitatively, quickly and locally in a live organism. Here, we present the experimental details behind this approach.