On the relation between noise spectra and the distribution of time between steps for single molecular motors

Publication Type:

Journal Article

Source:

SINGLE MOLECULES, WILEY-V C H VERLAG GMBH, Volume 3, Number 1, PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY, p.43-48 (2002)

Keywords:

ATP HYDROLYSIS; ELASTICITY; KINETICS; MECHANISM; STEADY-STATE ANALYSIS; SUPERCOILED DNA MOLECULE; TOPOISOMERASE-II

Abstract:

A large class of molecular motors (kinesin, dynein, myosin, RNA-polymerase, helicase, F1-ATPase, etc.) consists of enzymes that proceed by successive steps of fixed size. We hereby address the relation between the statistics of the time between steps and the power spectrum of the enzyme's displacement in the presence of strong instrumental noise. From the recordings of the noisy signal of an enzyme, an algorithm is provided to deduce its step size and to characterize the distribution of its stepping probability.