Sensing single base incorporation with nanopore micromanipulation

Publication Type:

Journal Article

Source:

ACS CHEMICAL BIOLOGY, AMER CHEMICAL SOC, Volume 3, Number 2, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA, p.92-94 (2008)

DOI:

10.1021/68000205

Keywords:

POLYMERASE; POLYNUCLEOTIDE MOLECULES

Abstract:

Using single-molecule techniques, scientists can routinely investigate the action of a single enzyme. The goal of such studies is usually to gain accurate information unachievable in ensemble assays, such as the maximum instantaneous rate of reaction, the existence of pauses or backward steps, etc. In the article discussed here, the authors have increased their experimental sensitivity so that they can detect a single enzymatic cycle. This improvement makes possible the use of a polymerase enzyme to sequence a single DNA molecule.