Single DNA/protein studies with magnetic traps

Publication Type:

Journal Article

Source:

CURRENT OPINION IN STRUCTURAL BIOLOGY, Volume 19, Number 5, p.615-622 (2009)

Abstract:

Magnetic traps provide a simple technique to pull and twist a variety of biomolecules and monitor the resulting change in extension. They have been used with great success to investigate the interaction of stretched and supercoiled DNA and DNA fibers (e.g. chromatin) with a great variety of enzymes. In this small review we will address their recent use in the study of topoisomerases, gyrase, DNA translocases and various structural proteins.