Morphogenesis of bacterial microcolonies

Surfaces are privileged area where bacteria can attach for initiating the basement of biofilm formation. In this process, the development of a micro-colony from an adherent cell is a key step before biofilm maturation.

Since rod-shaped bacteria elongate along their main axis, we would expect the symmetry of the colony to reflect the symmetry of its elementary components. However on solid substrates microcolonies start to form disks at very early stages of development. We study the morphogeneis of micro-colonies thanks to laser ablation (to probe single cell adhesion) and Traction Force Microscopy (to probe adhesion inside micro-colonies).

Fig1: Ablation of daughter cells

Fig2: Traction Force Microscopy