Control of protein activity and gene expression by cyclofen-OH uncaging.

Publication Type:

Journal Article


Chembiochem : a European journal of chemical biology (2018)




The use of light to control the expression of genes and the activity of proteins is a rapidly expanding field. While many of these approaches use a fusion between a light activable protein and the protein of interest to control the activity of the later, it is also possible to control the activity of a protein by uncaging a specific ligand. In that context, controlling the activation of a protein fused to the modified estrogen receptor (ERT) by uncaging its ligand cyclofen-OH has emerged as a generic and versatile method to control the activation of proteins quantitatively, quickly and locally in a live organism. We here present that approach and its uses in a variety of physiological contexts.